Sounding spectral meter of fluorescence and light scattering: laboratory and field testing
https://doi.org/10.7868/S2073667320020100
Abstract
In order to conduct environmental monitoring and obtain operational data on the state of the investigated water area, a sounding spectral fluorescence and scattering meter was created. It allows simultaneous measurements of the fluorescence of phytoplankton, dissolved organic matter and light scattering in the sea water, in the spectral range covering the region from the near ultraviolet to the red boundary of the visible spectrum in the same measuring volume.
The results of instrument testing in laboratory conditions on samples of phytoplankton monocultures existing in the Black Sea waters showed good agreement between the measurements and literature data. During the expedition of the R/V “Professor Vodyanitsky”, at 77 hydrological stations the following parameters were measured: vertical profiles of the phytoplankton fluorescence; DOM; the elastic light scattering. As a result, information was obtained on the vertical distribution of the measured parameters in the studied waters of the Black Sea. The results of tests of the presented meter allow to approve the technical design and works methodology of this meter.
Keywords
spectral fluorometer,
microalgae fluorescence excitation spectrum,
chlorophyll,
DOM,
vertical fluorescence profiles,
Black Sea
Supporting agencies: This work was carried out as part of a state assignment No. 0827–2018–0002 (code “Operational Oceanology”) and No. 0827–2018–0006.01 (code “Biooptics”) and on the topic “Functional, metabolic and toxicological aspects of the existence of aquatic organisms and their populations in biotopes with different physicochemical conditions”; State registration number AAAA-A18–118021490093–4.
About the Authors
O. B. Kudinov
Marine Hydrophysical Institute RAS
Russian Federation
Russian Federation
299011, Kapitanskaya Str., 2, Sevastopol
O. V. Martynov
Marine Hydrophysical Institute RAS
Russian Federation
Russian Federation
299011, Kapitanskaya Str., 2, Sevastopol
R. I. Lee
A.O. Kovalevsky Institute of Biology of the Southern Seas RAS
Russian Federation
Russian Federation
299011, Nakhimova Prospekt, 2, Sevastopol
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Review
For citations:
Kudinov O.B., Martynov O.V., Lee R.I. Sounding spectral meter of fluorescence and light scattering: laboratory and field testing. Fundamental and Applied Hydrophysics. 2020;13(2):82-87. https://doi.org/10.7868/S2073667320020100
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